LCMS detection methods for cyanotoxins in tissue and environmental samples from brackish and freshwater water bodies
Gdansk, Russian federation
We detected saxitoxins in 6 among12 biomass samples from reservoirs of Central part of Russia, which is in agreement with phytoplankton composition and previously obtained data of PCR analysis.
8 microcystins varints, anatoxin-a, cylindrospermopsin? and cyanopeptolins, anabaenopeptins were identified in biomass samples.
No cyanotoxins were detected in analyzed tissue samples from Curonian Lagoon, northwestern part of Russia.
Application of hydrogen peroxide in order to control the cyanobacteria blooming
Three experiments wer done in this STSM.
1. Application of hydrogen peroxide on the water samples from Lake köyliönjärvi.
2. Sample preparation and determination of microcystin concentraions in the animal and plant tissues from Serbia.
3. Identification and quantification of cyanotoxines in water samples taken from Ludas Lake (Serbia).
Distribution and toxicity of cyanobacteria in Nordic and Mediterranean lakes and water reservoirs
The purpose of the STSM was to analyze the distribution and compare the habitat ecology of invasive cyanobacteria and Nordic lakes and water reservoirs.
the invasion od Dolichospermum Lemmermannii in the large lakes south of the alps : characterization of genotypes and colonization rates in the European context
Creation of AACC (Åbo Akademi Culture Collection)
Creation of culture collection named AACC (Åbo Akademi Culture Collection) that represents an
important way of biodiversity preservation of a specific region, but also it offers the possibility of
target selection and use of microorganisms in various fields of science and technology.
Characterization of the MC degradation process by MC-degrading bacteria from a Spanish reservoir
There are several constrictions related to the removal and/or inactivation of microcystins by physic and
chemical processes (Westrick et al., 2010). However, it is known that heterotrophic bacteria from water blooms
and sediments are able to degrade microcystins (Ishii et al., 2004), offering a real potential tool for toxin
removal (Ho et al., 2007).
Cyanobacterial oligopeptides isolation and structure-activity studies
Cyanobacteria are a rich source of secondary metabolites which show diverse structure and biological activity. Some of
the metabolites play an important ecological role in interactions with aquatic biota. Other compounds have
biotechnological application. Peptides belong to the most widely studied group of cyanobacterial metabolites. Many of
the compounds belong to inhibitors of thrombin (e.g. aeruginosins), trypsin (e.g. cyanopeptoline), elastase and
chymotrypsin (e.g. planktopeptin) and carboxypeptidase A (e.g. anabaenopeptins). Enzymes play central role in the
human organism. Naturally produced inhibitors of serine proteases are considered to be potential therapeutic agents in
cardiovascular disorders, thrombosis, viral infections and other health problems.
The aim of study was isolation of anabaenopeptins produced by the Baltic cyanobacteria. In this work, the biological
activity of anabaenopeptins was tested in enzymatic assays.
Know-How Exchange Finland-Serbia
The purpose of this STSM was to transfer some critical parts of the acquired knowledge and
technological know-how from Åbo Akademi University/Jussi Meriluoto to University of Novi
Sad/prof. Zorica Svircev’s group. The applicant in turn generally benefited from the vast
experience of prof. Svircev related to health effects of toxic cyanobacteria, management of
cyanoproblems, cyanobacterial taxonomy and cyanobacterial culturing.
Seasonal variation of cyanotoxins and toxic genotypes detection of cyanobacteria in hypertrophic lake
1. Determination and quantification of microcystins in environmental samples and cyanobacteria strains using LC–MS technique.
2. Identification and quantification of genes responsible for microcystins, saxitoxins, anatoxins synthesis in environmental samples and cyanobacteria strains.
Bioactive properties of cyanobacteria and microalgae isolated from brackish rock pools
Enzyme inhibition and antibacterial properties of crude extracts of rock pool cyanobacteria and
microalgae were studied. The crude extracts were analysed with liquid chromatography-mass
spectrometry (LC-MS) and genetic studies of the cyanobacteria were performed. The work is part of a PhD-thesis.