Short Term Scientific Missions

STSM posters

“Cyanotoxins: Sample preparation and determination by LC-MS and HPLC-DAD"

by Vera Pavlova

The general purposes of this STSM are:

1.Training in determination of cyanotoxins;
2.Sample collection and sample preparation;
3.Determination of cyanotoxins in samples from Bulgarian water bodies.

Evaluation of the efficiency of Advanced Oxidation Processes (AOPs) for the removal of various microcystins (MCs) under realistic conditions

The aim of this study was to compare the efficiency of different Advanced Oxidation Processes (AOPs) for the removal of a mixture of cyanotoxins under realistic conditions (MC concentration and water matrix) and to determine which is the most efficient treatment. Three derivatives from the group of microcystins (MC-LR, MC-YR, MC-RR) were chosen as model cyanotoxins because of their frequency of appearance and because studies have shown that a bloom can produce up to 12 different types of cyanotoxins. The study also aimed to identify and quantify the effects of different water parameters have on the removal efficiency of MCs and, were applicable, the percentage of inhibition of each parameter. Bottled water was used to simulate the water matrix under realistic conditions. Since the “Catalytic-Photocatalytic Processes Laboratory (Solar Energy, Environment) and Environmental Analysis Laboratory” of NCSR Demokritos is one of the few facilities in the world that have accredited methods on the detection of microcystins with quantification limit in the ng/L range, it was agreed to perform the experiments there.

Tracing toxigenic cyanobacteria from preserved genetic materia

by Veronica Ostermaier
Innsbruck, Oslo

Following eutrophication of Lake Gjersjøen (Norway),  dense surface blooms of a green-pigmented ecotype of the cyanobacterium Planktothrix were observed  starting in 1964. After the initial  operation of a sewage treatment plant in 1971 the trophic state  improved to meso-/eutrophic. With the increasing N/P ratio (Fig. 1) a red-pigmented Planktothrix ecotype that formed metalimnetic blooms became dominant (Fig. 2). This red-pigmented variant had first been observed in summer 1970 and seemed to have displaced the green-pigmented ecotype after 1971 (Faafeng&Nilssen).

ATX genes in environmental samples and isolated strains: towards the identification of new producers

by Shiva Shams
Helsinki , San Michele all’Adige

Detect the populations of cyanobacteria producing Anatoxin-a (ATX) in a deep, oligotrophic lake (Lake Garda, NE Italy). Find out the main microcytin (MC) producers in this lake.

On the way to develop molecular methods for the monitoring of emerging cylindrospermopsin (CYN) producers in Europe

by Samuel Cires
Helsinki , Madrid

To develop a molecular method for the detection of CYN-producing Anabaena in field samples, based on selective amplification CYN biosynthesis genes (cyrA, cyrB, cyrC and cyrJ). The STSM focused on optimizing of the method in cyanobacterial cultures, making it available for future tests with water samples potentially containing CYN-producing Anabaena spp.

Evaluation of the efficiency of Advanced Oxidation Processes (AOPs) for the removal of various microcystins (MCs) under realistic conditions

by Maria Antoniou

BMAA accumulation by blue mussel fed with cyanobacteria from the Baltic Sea

by Mafalda Baptista
Porto / no informations, Stockholm / no informations

This work aimed at studying the kinetic of BMAA accumulation and detoxification in blue mussel Mytilus edulis fed with Baltic Sea cyanobacteria-blooming species

Molecular detection methods for potentially toxic cyanobacteria in freshwater lakes

by Kristel Panksep
Helsinki , Tartu

The aim of the STSM was to acquaint myself with new experimental and laboratory methods concerning molecular and analytical studies of toxic cyanobacteria. STSM was focused mainly on molecular detection and identification methods of potential microcystin and anatoxin-a producing cyanobacteria in environmental samples.

Microcystis RNA Profiling

by Katharina Makower

The STSM at the University of Amsterdam aimed at getting new insights into transcriptional profiling of different cyanobacterial Microcystis aeruginosa strains. Especially, the reaction to external factors in Microcystis’ environment, such as microcystin and other cyanobacterially produced compounds, and the potential implications in its ecological success were in focus. These experiments represent a major part of a PhD theses.

Autoregulation of nodularin biosynthesis in the cyanobacterium Nodularia spumigena ?

by Anna Torunska
Gdansk, Helsinki

The purpose of the STSM project was to continue the studies on the regulation of the nodularin (NOD) biosynthesis in the cyanobacterium N. spumigena using advanced molecular technique (RT-qPCR)